Nature Methods – Application Notes

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Nature Methods – Application Notes

Featured Application Note

DNA decontamination: DNA-ExitusPlus in comparison with conventional reagents

http://www.applichem.de >Full text

Here we present a completely new DNA decontamination reagent, DNA-ExitusPlus. In comparison with conventional products, DNA-ExitusPlus guarantees fast and efficient destruction of nucleic acids without corrosive or toxic properties. DNA-ExitusPlus was developed by AppliChem GmbH, Darmstadt, in cooperation with multiBIND biotec GmbH, Dortmund.

Cisbio International

IP-One assay: the new solution for GPCR investigation

http://www.cisbiointernational.fr >
PDF (241K)

Cisbio international has developed a complete cell-based platform to investigate G protein?coupled
receptor (GPCR) activity. This article describes the three-step process to monitor GPCR activity using the
Cisbio IP-One kit.

Dharmacon RNA Technologies

siARRAY Reverse Transfection Format (RTF): a rapid method for RNAi-based high-throughput studies of biological pathways

http://www.dharmacon.com >PDF (185K)

RNA interference (RNAi) screens are easier and more efficient with Dharmacon?s siARRAY RTF small
interfering RNA (siRNA) libraries, which combine optimized broad-spectrum transfection reagents and
potent SMARTpool® siRNAs into simple, assay-ready and automation-compatible formats.

Leica Microsystems

True confocal resonant scanning: a better solution for high-speed multifluorescence optical sectioning

http://www.leica-microsystems.com >PDF (267K)

Single-beam confocal imaging at high speed, using Leica Microsystems? new TCS SP5 system,
outperforms parallel scanning approaches. The result is clearly superior performance, with better
resolution and the applicability of multiple-labeled samples, a higher signal-to-noise ratio and
lower photobleaching.

Olympus Biosystems

Cell-based genome-wide RNAi screening with the new scan^R Screening Station

http://www.olympus-europa.com/microscopy/Scan_R/ >PDF (424K)

In collaboration with the European Molecular Biology Laboratory (EMBL), Olympus has developed a
fully automated and highly flexible microscope-based screening platform for life science applications.

Pierce Endogen

Quantitative measurement of proteins using
SearchLight® chemiluminescent, infrared and fluorescent arrays

http://www.perbio.com >PDF
(166K)

Using SearchLight Multiplex Protein Arrays, researchers continue to obtain quantitative
chemiluminescent data for a wide range of biomarkers in their samples. With the addition of the
new infrared and fluorescent formats, researchers with access to infrared and fluorescent scanning
instrumentation can experience the benefits of multiplex protein quantification.

MMI & AmpTec

Laser microdissection of a colon cancer specimen with subsequent RNA isolation, mRNA amplification and microarray hybridization

http://www.molecular-machines.com >
http://www.amp-tec.com >
PDF (243K)

Laser microdissection with the MMI Cellcut® allows the efficient isolation of specific cells. The collected cells yield only tiny amounts of isolated RNA that require dedicated mRNA amplification technology, and
ExpressArt® from AmpTec offers the unique possibility to exploit the complete samples, irrespective of RNA
amounts and without impairing sample comparability.

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